Abstract

Traditional microscopes do not meet a wide field of view and high resolution at the same time. We propose a method for image restoration and color fusion of digital microscopes. It combines a single high-resolution gray scale hologram with a low-resolution color image to obtain a high-resolution color image. Specifically, the high-resolution gray scale image is obtained by reconstructing three different height holograms using a wavelet-based method, and the color information is obtained using a portable cell phone microscope. The subsequent calibration and blending of colors ultimately results in a high-resolution, wide-field color map that can be of great help in the study of pathology or biomedicine. This method breaks the rule that the large field of view and high resolution of a traditional microscope cannot be simultaneously satisfied, which can realize a more comprehensive observation of the shape and details of the slice.

© 2019 Optical Society of America

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