Abstract

We present autofluorescence of six zooplankton species, including salmon lice (Lepeophtheirus salmonis), for the purpose of classification in marine environments. Using a 410 nm excitation wavelength, we find that all measured zooplankton species exhibit broad cyan fluorescence centered around 510–520 nm. Furthermore, salmon lice show an absence of red fluorescence from undigested chlorophyll, which is measured from the gut of the herbivorous zooplankton species. We show the capability to distinguish noneating species, including salmon lice, from algae-eating species using a dual-band analysis of the fluorescence spectra. This shows the potential of autofluorescence as an important signature in real-time monitoring and classification of salmon lice.

© 2019 Optical Society of America

Dual-band fluorosensor for discriminating non-eating from algae-eating zooplankton in aquatic environments

J. H. Nielsen, C. Pedersen, T. Kiørboe, T. Nikolajsen, M. Brydegaard, and P. J. Rodrigo
OSA Continuum 3(7) 1730-1738 (2020)

Comparison of airborne lidar measurements with 420 kHz echo-sounder measurements of zooplankton

James H. Churnside and Richard E. Thorne
Appl. Opt. 44(26) 5504-5511 (2005)

Airborne lidar imaging of salmon

James H. Churnside and James J. Wilson
Appl. Opt. 43(6) 1416-1424 (2004)

Inelastic hyperspectral Scheimpflug lidar for microalgae classification and quantification

Xiang Chen, Yiming Jiang, Quankai Yao, Jiali Ji, Julian Evans, and Sailing He
Appl. Opt. 60(16) 4778-4786 (2021)

General analysis and optimization strategy to suppress autofluorescence in microscope lenses

Xiang Lu, Yueqian Zhang, and Herbert Gross
Appl. Opt. 58(27) 7404-7415 (2019)