Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Immunosensing platform with large detection range using an excessively tilted fiber grating coated with graphene oxide

Not Accessible

Your library or personal account may give you access

Abstract

We report an immunosensing platform with a large detection range using an excessively tilted fiber grating (ExTFG) coated with graphene oxide (GO). ExTFG was inscribed in standard single-mode fiber; GO film was coated on the fiber surface through hydrogen bond. The effectiveness and uniformity of GO deposited on the ExTFG surface were investigated by field emission scanning electron microscopy and energy spectrum method. Bovine serum albumin (BSA) monoclonal antibodies (MAbs) were used as biometric units to link the GO film through a covalent bond for the specific detection of BSA, so as to evaluate the performances of the proposed biosensor. The whole dynamic immobilization process of BSA MAbs and BSA detection were observed by the spectral evolution of the sensor. Experimental results show that the fabricated GO-coated ExTFG biosensor has a large detection range from 1.5 nM–75 nM and fast response for BSA antigen; the limit of detection is 0.88nM by using an optical spectrum analyzer with a resolution of 0.03 nm, and the dissociation constant KD and the affinity constant KA are calculated to be 6.66×109M and 1.5×108M1, respectively. The proposed GO-coated ExTFG immunosensing platform could lay a foundation for the specific detection of other biomolecules.

© 2018 Optical Society of America

Full Article  |  PDF Article
More Like This
Label-free and specific detection of soluble programmed death ligand-1 using a localized surface plasmon resonance biosensor based on excessively tilted fiber gratings

Binbin Luo, Yajie Wang, Huafeng Lu, Shengxi Wu, Youming Lu, Shenghui Shi, Lingchen Li, Shanghai Jiang, and Mingfu Zhao
Biomed. Opt. Express 10(10) 5136-5148 (2019)

Microfluidic immunosensor based on a graphene oxide functionalized double helix microfiber coupler for anti-Müllerian hormone detection

Yujie Li, Binbin Luo, Yanan Liu, Sehngxi Wu, Shenghui Shi, Huiji Chen, and Mingfu Zhao
Biomed. Opt. Express 14(4) 1364-1377 (2023)

Human heart failure biomarker immunosensor based on excessively tilted fiber gratings

Binbin Luo, Shengxi Wu, Zhonghao Zhang, Wengen Zou, Shenghui Shi, Mingfu Zhao, Nianbing Zhong, Yong Liu, Xue Zou, Lingling Wang, Weina Chai, Chuanmin Hu, and Lin Zhang
Biomed. Opt. Express 8(1) 57-67 (2017)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (8)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (1)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved