Abstract

Uncertainties in the microscopical measurement of small spheres as influenced by index of refraction of the mount, focus, aperture, and resolution have been variously reported and denied. The present study was conducted with large spheres in order to disclose the basic phenomena, but with such optical conditions as would ordinarily be used for much smaller objects. The reality of each reported error is verified. The principal uncertainties originate in the geometrical optics of thick bodies, but these effects tend to be confused by limitations in resolving power.

© 1965 Optical Society of America

An Integrating Sphere System for Measuring Average Reflectance and Transmittance

John M. Davies and Walter Zagieboylo
Appl. Opt. 4(2) 167-174 (1965)

Optical Instrumentation for the Biologist: Microscopy

Jurgen R. Meyer-Arendt
Appl. Opt. 4(1) 1-9 (1965)

Electron Microscope Method for Measuring Diffraction Grating Groove Geometry

W. A. Anderson, G. L. Griffin, C. F. Mooney, and R. S. Wiley
Appl. Opt. 4(8) 999-1003 (1965)

A Laser Microscope

N. A. Peppers
Appl. Opt. 4(5) 555-558 (1965)

Scattering Properties of Concentric Soot–Water Spheres for Visible and Infrared Light

Robert W. Fenn and Hansjörg Oser
Appl. Opt. 4(11) 1504-1509 (1965)