Abstract

Digital imaging systems intended for use in quantitative cell image analysis must meet stringent requirements on both photometric and spatial resolution to support the type of investigations currently ongoing in the biomedical research community. There must be a match between the optics (microscope), the image sensor (camera), the image digitization and storage hardware, and the algorithms for quantitative analysis of the digitized images. Applicable theory from the fields of optics, electronics, statistics, and signal processing allows development of workable rules for designing such a balance.

© 1987 Optical Society of America

Optical microscope system for standardized cell measurements and analyses

James W. Bacus and Les J. Grace
Appl. Opt. 26(16) 3280-3293 (1987)

Accuracy of optical density measurement of cells. 1: Low resolution

A. W. M. Smeulders and T. K. ten Kate
Appl. Opt. 26(16) 3249-3257 (1987)

Automated densitometry of cell populations in a continuous-motion imaging cell scanner

James H. Tucker, O. A. N. Husain, Keith Watts, Stephen Farrow, Royston Bayley, and Margaret H. Stark
Appl. Opt. 26(16) 3315-3324 (1987)

Automated image analysis in the diagnosis of bladder cancer

Karl K. Melder and Leopold G. Koss
Appl. Opt. 26(16) 3367-3372 (1987)

High performance pathology workstation using an automated multispectral microscope

Harvey L. Kasdan, Kenneth C. Langford, John Liberty, Michael Zachariash, and Fred H. Deindoerfer
Appl. Opt. 26(16) 3294-3300 (1987)