Abstract

Application of spatial frequency filtering techniques gives powerful tools for the automation of screening of biomedical microsamples. Band-pass filtering provides a size selection of objects, whereas high-pass filtering combined with a subsequent reduction of the field size enables the separation of the superposition of the field and the object spectrum. Besides the theoretical background and numerical evaluations, experimental applications to cytological and cytogenetical samples are shown.

© 1977 Optical Society of America

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