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Diffusion constant measurements of macromolecules in solutions containing particulate components

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Abstract

The analysis and measurement of the dynamic properties of a monodispersed macromolecule in a solution containing a distribution of particulate contaminants are reported. The resulting measured diffusion constants have values that agree with the accepted values found by ultracentrifuge methods. The inclusion in the analysis of a simple approximation of the effects of the contaminant background eliminates the necessity of ultrapure samples, which are often impractical or impossible to prepare.

© 1975 Optical Society of America

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