Abstract

When a spectrophotometer with the correct optical configuration is used to measure a fluorescent sample, the resulting spectral radiance factor curve consists of the sum of a reflected and a fluoresced portion. The true reflectance curve can be obtained by direct measurement below the emission region and also above the absorption region by the use of a fluorescence-killing filter. Between these two limits, in the region of simultaneous absorption and emission (the crossover region), the true reflectance curve can be obtained by calculation from two different spectral radiance factor curves and a single reading through the fluorescence-killing filter.

© 1973 Optical Society of America

Separating the fluorescence and reflectance components of coral spectra

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Mode of Action of Fluorescent Whitening Agents and Measurement of Their Relative Efficiency

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Spectral Radiance in the S20-Range and Luminance of the Clear and Overcast Night Sky

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Design and testing of a two-monochromator reference spectrofluorimeter for high-accuracy total radiance factor measurements

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