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Simultaneous Selective Two-Photon Microscopy Using MHz Rate Pulse Shaping and Quadrature Detection of the Time-Multiplexed Signal

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Abstract

We demonstrate a method for simultaneous fast selective two-photon excited fluorescence (TPEF) microscopy imaging of two different fluorophores using quadrature detection of the signal from a single PMT detector.

© 2014 Optical Society of America

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