Abstract
Two-photon excitation, which avoids the harmful biological effects of ultraviolet exposure and provides intrinsic axial resolution, has been widely used in confocal microscopes to image static structures in biological samples. We have combined two-photon excitation with patterned photobleaching and a standing wave geometry in order to study macromolecular dynamics in such samples. The aim of this work is to measure the short-range diffusive motion of the DNA-containing chromatin in live cells, which is believed to be important in biological gene expression. Conformational fluctuations of the densely packed chromatin fibers are thought to allow transcription proteins access to buried sequences of DNA in the nucleoplasm.
© 2002 Optical Society of America
PDF ArticleMore Like This
Christopher J. Bardeen, Kerry M. Hanson, and Sara K. Davis
JMD3 Frontiers in Optics (FiO) 2007
M. Tomas, P. Blumhardt, A. Deutzmann, D. Kromm, A. Leitenstorfer, and E. Ferrando-May
CTu2M.6 CLEO: Science and Innovations (CLEO:S&I) 2013
Ji-Xin Cheng and X. Sunney Xie
MC4 International Conference on Ultrafast Phenomena (UP) 2002