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  • The Thirteenth International Conference on Ultrafast Phenomena
  • 2002 OSA Technical Digest Series (Optica Publishing Group, 2002),
  • paper ThA6
  • https://doi.org/10.1364/UP.2002.ThA6

Two-photon standing wave patterned photobleaching to probe DNA dynamics in live cells

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Abstract

Two-photon excitation, which avoids the harmful biological effects of ultraviolet exposure and provides intrinsic axial resolution, has been widely used in confocal microscopes to image static structures in biological samples. We have combined two-photon excitation with patterned photobleaching and a standing wave geometry in order to study macromolecular dynamics in such samples. The aim of this work is to measure the short-range diffusive motion of the DNA-containing chromatin in live cells, which is believed to be important in biological gene expression. Conformational fluctuations of the densely packed chromatin fibers are thought to allow transcription proteins access to buried sequences of DNA in the nucleoplasm.

© 2002 Optical Society of America

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