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Intracellular water diffusion probed by femtosecond nonlinear CARS microscopy

Eric O. Potma, Wim P. de Boeij, and Douwe A. Wiersma

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Abstract

We report on a nonlinear coherent anti-Stokes Raman microscope system based on a high repetition rate femtosecond cavity-dumped visible optical parametric oscillator. This microscope enables real-time mapping of water concentration gradients in single living cells at high spatial resolution.

© 2000 Optical Society of America

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