A protocol of polarization gating that combines co- and counter-elliptical measurements of in vivo samples illuminated at different wavelengths to selectively access sub-surface tissue layers and spectral information is proposed. To selectively access subsurface layers, the samples were illuminated with different wavelengths (532, 570 and 633nm) and processed using subtraction (for removal of multiple-scattered components) involving images at these wavelengths. In vivo experiments were performed on skin abnormalities of volunteers to confirm the results of the subtraction method and access spectral information.

© 2016 Optical Society of America

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