The study of endogenous fluorescence appears as the future of medical imaging, coupled with a two photon excitation it could lead to numerous medical progresses, especially in early diagnosis. However due to the lack of miniaturized two photon technology it’s still a largely unknown field. As we project to build such a device, we also study the endogenous response of human brain biopsy under a two-photon excitation. The samples were excited with wavelengths between 700 and 980 nm and the spectral responses were measured. Two types of samples were study, thin and thick ones, the thick biopsy had a broader spectrum at each wavelength. Moreover three interesting wavelength were found, 750 nm that excite the most NADH molecules, 850 nm were we measure second harmonic generation (SHG) and a fluorescence were FAD molecules are predominant and 890 nm were SHG is the dominant response.
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