Abstract
Interpreting the biochemical specificity of healthy spinal cord tissue is the essential requirement for understanding the biochemical mechanisms during spinal-cord-related pathological course. In this work, a longitudinal study was implemented to reveal a precise linkage between the spectral features and the molecular composition in ex vivo mouse spinal cord tissue by microspectral Raman imaging. It was testified that lipid-rich white matter could be distinguished from grey matter not only by the lipid Raman peaks at 1064, 1300, 1445 and 1660cm−1, but also by protein (1250 and 1328cm−1) and saccharides (913 and 1137cm−1) distributions. K-mean cluster analysis was further applied to visualize the morphological basis of spinal cord tissue by chemical components and their distribution patterns. All the obtained results illustrated the biochemical characteristics of spinal cord tissue, as well as some specific substances variances between different tissue types, which form a solid basis for the molecular investigation on pathology states of spinal cord injury.
© 2017 Optical Society of America
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