Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Characterization the biochemical specificity of mouse spinal cord by confocal Raman microspectral Imaging

Not Accessible

Your library or personal account may give you access

Abstract

Interpreting the biochemical specificity of healthy spinal cord tissue is the essential requirement for understanding the biochemical mechanisms during spinal-cord-related pathological course. In this work, a longitudinal study was implemented to reveal a precise linkage between the spectral features and the molecular composition in ex vivo mouse spinal cord tissue by microspectral Raman imaging. It was testified that lipid-rich white matter could be distinguished from grey matter not only by the lipid Raman peaks at 1064, 1300, 1445 and 1660cm−1, but also by protein (1250 and 1328cm−1) and saccharides (913 and 1137cm−1) distributions. K-mean cluster analysis was further applied to visualize the morphological basis of spinal cord tissue by chemical components and their distribution patterns. All the obtained results illustrated the biochemical characteristics of spinal cord tissue, as well as some specific substances variances between different tissue types, which form a solid basis for the molecular investigation on pathology states of spinal cord injury.

© 2017 Optical Society of America

PDF Article
More Like This
Confocal Raman microscpectral imaging of human spinal cord sections employing uni- and multi-variate methods for data analysis

Shuang Wang, Kaige Wang, Zhuowen Liang, Yuze Gong, Xueyu Hu, Yaning Yin, Qingli He, Zhe Wang, and Jintao Bai
JTu5A.9 CLEO: Applications and Technology (CLEO:A&T) 2017

Raman-based imaging uncovers the effects of alginate hydrogel implants in spinal cord injury

Roberta Galli, Sandra Tamosaityte, Maria Koch, Kerim H. Sitoci-Ficici, Robert Later, Ortrud Uckermann, Rudolf Beiermeister, Michael Gelinsky, Gabriele Schackert, Matthias Kirsch, Edmund Koch, and Gerald Steiner
95360Y European Conference on Biomedical Optics (ECBO) 2015

In-Vivo Three-Photon Excited Fluorescence Imaging in the Spinal Cord of Awake, Locomoting Mouse

Y.T. Cheng, S.L. Ness, S.H. Hu, J. Raikin, L.D. Pan, T. Wang, D.G. Ouzounov, J.C. Cruz-Hernandez, I.M. Bastille, N. Nishimura, J.R. Fetcho, C. Xu, and C.B. Schaffer
JTh2A.183 Frontiers in Optics (FiO) 2016

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved