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Measuring Structural, Chemical, and Biomechanical Properties of Live Amphibian Erythrocytes Using Optical Diffraction Tomography

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Abstract

We present optical characterizations of live amphibian erythrocytes by utilizing quantitative phase imaging. Without any labeling or preparation procedures, 3-D refractive index tomogram and 2-D phase delay of amphibian erythrocyte were measured using Mach-Zehnder interferometry. Refractive index value in 3-D tomograms represent structural and chemical features of an erythrocyte and the nucleus. In addition, membrane fluctuation of amphibian erythrocytes was measured to be more profound than that of human erythrocytes.

© 2017 Optical Society of America

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