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SPEED Microscopy: Fast Single-molecular Tracking and 3D Deconvolution Process

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Abstract

For studying the Nucleocytoplamic transport mechanism, we developed a new methods named single-point edge-excitation sub-diffraction (SPEED) microscopy. SPEED microscopy allows 3D spatial density maps of molecular pathway of the NPC with a spatiotemporal resolution of 9 nm and 400 μs.

© 2017 Optical Society of America

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