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Probing the Elasticity of Short Proteins with Optical Tweezers

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Abstract

Probing relatively short molecules, such as the protein elastin (~200 nm), with optical tweezers requires manipulating trapped polystyrene beads at separations small compared to their micron dimensions. We discuss experimental complications arising from this proximity, such as hydrodynamic and optical interactions, and our efforts to minimize them.

© 2009 Optical Society of America

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