using nylon 6 nanofiber membrane prepared by self-made electrospinning device as absorbing material, a method for rapid determination of trace polycyclic aromatic hydrocarbons (PAHs) in water by solid-phase surface spectrofluorimetry has been established. Nylon 6 nanofiber membranes with a diameter of 5cm were used for the filtration of three kinds of PAHs (phenanthrene, pyrene, fluoranthene) solution, then the membranes were moved to the variable angle powder sample pool after air-drying, and three dimensional excitation-emission solid-phase surface fluorescence spectra of PAHs which was absorbed on the membrane was determined using fluorescence spectrophotometer. According to the three dimensional excitation-emission solid-phase surface fluorescence spectra, the excitation/emission wavelength pair with the maximum fluorescence intensity was found out and the linear relationship between fluorescence intensity and the initial concentration of each PAHs in water were investigated. The results show that: the excitation/emission wavelength pairs with the maximum fluorescence intensity located at Ex255nm/Em368nm, Ex340nm/Em376nm and Ex290nm/Em437nm for phenanthrene, pyrene, and fluoranthene respectively. In the situation of 500mL sample solution for suction filtration, the calibration curves between the fluorescence intensity and the initial concentration for three kinds of PAHs were listed as follow: y=9432.4x+261.1 with linear concentration range of 5~500 ng/mL for phenanthrene; y=753480x+805.51 with linear concentration range of 0.2~10 ng/mL for pyrene; and y= 9946.06x+603.48 with linear concentration range of 10 ~ 400 ng/mL for fluoranthene. The detection limits were 0.973ng/mL for phenanthrene, 0.0162 ng/mL for pyrene and 0.0896ng/mL for fluoranthene. For phenanthrene standard solution of 100 ng/mL, pyrene standard solution of 10 ng/mL and fluoranthene standard solution of 50 ng/mL, the relative standard deviations(RSD) for seven time determinations were 7.0%, 2.6% and 5.1% respectively, and relative errors of mean values were 1%, 2% and −0.2% respectively. For tap water containing 50 ng/mL, 200 ng/mL and 500 ng/mL phenanthrene, the average recoveries were 87.2%~98.2%; for tap water containing 0.5 ng/mL, 2 ng/mL and 10 ng/mL pyrene, the average recoveries were 101%~120% and for tap water containing 10 ng/mL, 50 ng/mL and 100 ng/mL fluoranthene, the average recoveries were 85.8%~92.3%. Therefore, the method has the advantages of simplicity, economy, high sensitivity, which is suitable for the rapid identification and detection of trace polycyclic aromatic hydrocarbons in water.
© 2017 Optical Society of AmericaPDF Article