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  • 2017 OPJ-OSA Joint Symposia on Nanophotonics and Digital Photonics
  • OSA Technical Digest (online) (Optica Publishing Group, 2017),
  • paper 31pOD6

Monitoring of mitochondrial membrane potential by using two-photon fluorescence microscope

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Abstract

Here we describe the design and synthesis of a bifunctional two-photon fluorescence probe BP6. Live cells were stained with BP6. BP6 accumulated on the mitochondria of all three cell types when the mitochondrial membrane potential was high. As the mitochondrial membrane potential decreased, BP6 moved from the mitochondria to the nucleus in a reversible manner, depending on the mitochondrial membrane potential status. This change detected by two-photon fluorescence microscope.

© 2017 Optical Society of Japan

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