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Confocal microscopy without pinhole

George Barbastathis, Michal Balberg, and David J. Brady

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Abstract

Confocal microscopes achieve depth resolution by use of a pinhole. On-axis, in-focus point-source objects are imaged exactly inside the pinhole, and give maximal intensity. Out-of-focus objects, even on-axis, produce extended (blurred) images, and are filtered out by the limited aperture of the pinhole.

© 1999 Optical Society of America

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