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Interferometric rotating point spread function and its application in localization based super resolution imaging

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Abstract

Rotating point spread functions (PSF), such as the double helix (DH) PSF, are widely used in localization-based super-resolution imaging because of its large working depth range. We propose an interferometric DH PSF (iDH PSF) using two opposed objective lenses as in the 4Pi microscope. In the proposed iDH PSF, the super-resolution in the axial PSF is achieved in the azimuthal direction, and can be observed using the interference effect. Moreover, we design an iDH PSF whose imaging range reaches 3µm, around 3 times better than that can be obtained by using other interferometric localization-based super-resolution imaging methods.

© 2017 Optical Society of America

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