3D Phase Microscopy with Coded Illumination

Michael Chen; Laura Waller

doi:10.1364/NTM.2017.NM2C.2

Optics in the Life Sciences Congress
OSA Technical Digest (online) (Optica Publishing Group, 2017),
paper NM2C.2
•https://doi.org/10.1364/NTM.2017.NM2C.2

3D Phase Microscopy with Coded Illumination

Michael Chen and Laura Waller

Novel Techniques in Microscopy 2017

San Diego, California United States
2–5 April 2017
ISBN: 978-1-943580-25-5

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Phase Microscopy II (NM2C)

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M. Chen and L. Waller, "3D Phase Microscopy with Coded Illumination," in Optics in the Life Sciences Congress, OSA Technical Digest (online) (Optica Publishing Group, 2017), paper NM2C.2.

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Abstract

We demonstrate 3D phase reconstruction from through-focus intensity images taken in an LED array microscope. Using the first Born and weak object approximations, 3D refractive index can be linearly related to measured intensity.

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