Multiphoton microscopy of cleared human tissue for 3D histology

Michael J. Levene; Kevin Zhou; Sam Vesuna; Richard Torres

doi:10.1364/NTM.2013.NT3B.2

Optics in the Life Sciences
OSA Technical Digest (online) (Optica Publishing Group, 2013),
paper NT3B.2
•https://doi.org/10.1364/NTM.2013.NT3B.2

Multiphoton microscopy of cleared human tissue for 3D histology

Michael J. Levene, Kevin Zhou, Sam Vesuna, and Richard Torres

Novel Techniques in Microscopy 2013

Waikoloa Beach, Hawaii United States
14–18 April 2013
ISBN: 978-1-55752-966-4

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Nonlinear II (NT3B)

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M. J. Levene, K. Zhou, S. Vesuna, and R. Torres, "Multiphoton microscopy of cleared human tissue for 3D histology," in Optics in the Life Sciences, OSA Technical Digest (online) (Optica Publishing Group, 2013), paper NT3B.2.

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Abstract

Multiphoton microscopy of optically cleared human biopsies can create a true 3D histopathology. We present recent advances in clearing protocols that reduce the total clearing time to <1 hour and enable effective nuclear staining.

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