Rapid Volumetric Two-photon Imaging with an Extended Depth of Field

Gabrielle Thériault; Gabrielle Thériault; Yves De Koninck; Yves De Koninck; Nathalie McCarthy

doi:10.1364/NTM.2013.NT1B.6

Optics in the Life Sciences
OSA Technical Digest (online) (Optica Publishing Group, 2013),
paper NT1B.6
•https://doi.org/10.1364/NTM.2013.NT1B.6

Rapid Volumetric Two-photon Imaging with an Extended Depth of Field

Gabrielle Thériault, Yves De Koninck, and Nathalie McCarthy

Novel Techniques in Microscopy 2013

Waikoloa Beach, Hawaii United States
14–18 April 2013
ISBN: 978-1-55752-966-4

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Nonlinear I (NT1B)

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G. Thériault, Y. De Koninck, and N. McCarthy, "Rapid Volumetric Two-photon Imaging with an Extended Depth of Field," in Optics in the Life Sciences, OSA Technical Digest (online) (Optica Publishing Group, 2013), paper NT1B.6.

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Abstract

We present a system that offers the ability to control the depth of field in two-photon imaging by introducing an axicon in the light path to extend the focal spot in the axial direction.

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