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Conferences > NTM > 2013 > NM2B > NM2B.2

The Double-Helix Microscope Enables Precise and Accurate Measurement of 3D Single-Molecule Orientation and Localization Beyond the Diffraction Limit

Mikael P. Backlund, Matthew D. Lew, Adam S. Backer, Steffen J. Sahl, Ginni Grover, Anurag Agrawal, Rafael Piestun, and W. E. Moerner

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Abstract

Failure to account for the asymmetric nature of fluorophore emission in single-molecule super-resolution microscopy can lead to large localization errors. We use the Double-Helix microscope to correct such mislocalizations while simultaneously extracting 3D position and molecular orientation.

© 2013 Optical Society of America

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Measuring the 3D Position and Orientation of Single Molecules Simultaneously and Accurately with the Double Helix Microscope

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