Providing CLARITY: 3D Imaging of Biomarkers in Tumor Samples

Yi Chen; Qi Shen; Yesim Gokmen-Polar; Sunil Badve; Laurie J. Goodman

doi:10.1364/MICROSCOPY.2018.MF3A.1

Biophotonics Congress: Biomedical Optics Congress 2018 (Microscopy/Translational/Brain/OTS)
OSA Technical Digest (Optica Publishing Group, 2018),
paper MF3A.1
•https://doi.org/10.1364/MICROSCOPY.2018.MF3A.1

Providing CLARITY: 3D Imaging of Biomarkers in Tumor Samples

Yi Chen, Qi Shen, Yesim Gokmen-Polar, Sunil Badve, and Laurie J. Goodman

Microscopy Histopathology and Analytics 2018

Hollywood, Florida United States
3–6 April 2018
ISBN: 978-1-943580-41-5

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Cellular Diagnostics (MF3A)

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Y. Chen, Q. Shen, Y. Gokmen-Polar, S. Badve, and L. J. Goodman, "Providing CLARITY: 3D Imaging of Biomarkers in Tumor Samples," in Biophotonics Congress: Biomedical Optics Congress 2018 (Microscopy/Translational/Brain/OTS), OSA Technical Digest (Optica Publishing Group, 2018), paper MF3A.1.

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Abstract

The CLARITY method was used to transform core needle biopsies from patients with breast cancer, into optically transparent tissues, followed by multiplexed immunostaining and 3D imaging of key biomarkers. This data was compared to conventional FFPE thin-section methods. Manual pathology scoring of the 3D images from CLARITY processed samples demonstrated concordant results when compared to a set of 2D images scored throughout a similar sized FFPE block from the same patient.

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