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Chronic Transcranial Fluorescence Imaging of Cortical Neurons with 3-Photon Microscopy

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Abstract

3-photon excitation permits fluorescence microscopy through the intact skull. We demonstrate 3-photon fluorescence imaging of neuronal structure and calcium activity in a chronic transcranial window preparation up to 300 μm into brain below the intact skull.

© 2018 The Author(s)

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