Fundamental Limits on Imaging the Orientational Dynamics of Dipole-Like Emitters

Oumeng Zhang; Matthew D. Lew

doi:10.1364/MATH.2019.MM3D.2

Imaging and Applied Optics 2019 (COSI, IS, MATH, pcAOP)
OSA Technical Digest (Optica Publishing Group, 2019),
paper MM3D.2
•https://doi.org/10.1364/MATH.2019.MM3D.2

Fundamental Limits on Imaging the Orientational Dynamics of Dipole-Like Emitters

Oumeng Zhang and Matthew D. Lew

Mathematics in Imaging 2019

Munich Germany
24–27 June 2019
ISBN: 978-1-943580-63-7

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O. Zhang and M. D. Lew, "Fundamental Limits on Imaging the Orientational Dynamics of Dipole-Like Emitters," in Imaging and Applied Optics 2019 (COSI, IS, MATH, pcAOP), OSA Technical Digest (Optica Publishing Group, 2019), paper MM3D.2.

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Abstract

Photon shot noise causes unavoidable bias in estimating the rotational motions of fluorescent molecules. We quantify the bias of common 2D and 3D methods for imaging orientation, showing that they perceive the same 3D motion differently.

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