Assessing Mitochondria by Laser Induced Autofluorescence and Photoacoustic Measurements: A Preliminary In Vitro Study

Chandavalli Ramappa Raghushaker; Subhash Chandra; Kapaettu Satyamoorthy; Krishna Kishore Mahato

doi:10.1364/FIO.2019.JW4A.95

Frontiers in Optics + Laser Science APS/DLS
OSA Technical Digest (Optica Publishing Group, 2019),
paper JW4A.95
•https://doi.org/10.1364/FIO.2019.JW4A.95

Assessing Mitochondria by Laser Induced Autofluorescence and Photoacoustic Measurements: A Preliminary In Vitro Study

Chandavalli Ramappa Raghushaker, Subhash Chandra, Kapaettu Satyamoorthy, and Krishna Kishore Mahato

Laser Science 2019

Washington, DC United States
15–19 September 2019
ISBN: 978-1-943580-67-5

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Poster Session IV (JW4A)

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C. R. Raghushaker, S. Chandra, K. Satyamoorthy, and K. K. Mahato, "Assessing Mitochondria by Laser Induced Autofluorescence and Photoacoustic Measurements: A Preliminary In Vitro Study," in Frontiers in Optics + Laser Science APS/DLS, OSA Technical Digest (Optica Publishing Group, 2019), paper JW4A.95.

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Abstract

Mitochondrion during stress experiences a considerable change at the protein level and assessing such changes may help in better understanding of its dynamics. Hence, as a preliminary study, an attempt was made to record fluorescence and photoacoustic signals of the mitochondrial fractions from HepG2 and SiHa cell lines using Laser Induced Fluorescence (LIF) and Photoacoustic Spectroscopy (PAS) measurements at 281 nm excitations. The outcomes of the study revealed higher LIF and PAS signal strengths for the samples from HepG2 than SiHa.

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