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Adaptive Optics in Deep Optical Sectioning Microscopy of Biological Samples

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Abstract

Non-linear optical microscopy has permitted biological imaging at depth within intact, and frequently living, biological samples, permitting a greater understanding of biological processes. However, as one images every more deeply into samples the focal spot of the imaging system increases leading to a loss of resolution and signal due to sample induced aberrations. Through the use of active optical elements these aberrations can be compensated for though the exact optical configuration and method of determining the aberration to be corrected plays a crucial role in the overall performance of such system. The considerations in implementing a practical system and in particular the selection of the most appropriate aberration correction are discussed in detail.

© 2010 Optical Society of America

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