Single Molecule Fluorescence Correlation Spectroscopy in an Electrophoretic Mobility Shift Assay

Dale J. LeCaptain; Alan Van Orden

doi:10.1364/LACEA.2002.ThA2

Laser Applications to Chemical and Environmental Analysis
OSA Trends in Optics and Photonics (Optica Publishing Group, 2002),
paper ThA2
•https://doi.org/10.1364/LACEA.2002.ThA2

Single Molecule Fluorescence Correlation Spectroscopy in an Electrophoretic Mobility Shift Assay

Dale J. LeCaptain and Alan Van Orden

Laser Applications to Chemical and Environmental Analysis 2002

Boulder, Colorado United States
ISBN: 1-55752-699-0

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Biological or Biomedical I (ThA)

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D. J. LeCaptain and A. Van Orden, "Single Molecule Fluorescence Correlation Spectroscopy in an Electrophoretic Mobility Shift Assay," in Laser Applications to Chemical and Environmental Analysis, A. Sawchuk, ed., Vol. 69 of OSA Trends in Optics and Photonics (Optica Publishing Group, 2002), paper ThA2.

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Abstract

A capillary electrophoresis-based electrophoretic mobility shift assay is described wherein fluorescence correlation spectroscopy is used to resolve the bound and unbound fractions of a DNA-protein complex on the basis of their characteristic electrophoretic flow velocities.

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