Abstract

Optical microscopy possesses the capability to image the inside of biological tissues. Two-photon excitation microscopy achieved the spatial resolution in three dimensions by the nonlinear light-material interaction via two-photon excitation of fluorescent probes [1]. However, the contrast of image significantly degrades with increasing the observation depth since the signal light is attenuated during propagation through layers of the tissue before being detected.

© 2017 Japan Society of Applied Physics, Optical Society of America