Abstract

Raman scattering microscopy identifies molecular species in a sample by optically detecting molecular vibration, and provides spatial distribution of molecules in a specimen. Our developed slit-scanning Raman microscopy improved imaging speed more than 100 times faster than that of conventional point-scanning Raman microscopy, by providing both diffraction-limited high spatial resolution and chemical information. It allows us to observe dynamic behavior of biomolecules in a living cell during various biological processes, such as cytokinesis, mitosis, and apoptosis [1,2].

© 2015 Japan Society of Applied Physics, Optical Society of America