Abstract
Molecular organization at the submicron scale is of a great interest in crystals, bio-mineral structures or tissues, in which pathologies (skin lesion, neurodegenerative diseases, etc) can induce strong modifications in proteins’ organization. In this context, Second Harmonic Generation (SHG), Four Wave Mixing (FWM) and Coherent Raman Scattering (CARS), are powerful investigative tools to image specific objects without the need of labelling or staining. While numerous studies have been undertaken using polarization resolved second order nonlinear optical microscopy which is only specific to non-centrosymmetric organizations [1], higher order effects have been less explored. FWM microscopy and its resonant counterpart CARS can be of a great utility as label free diagnosis tools benefiting from less constraining symmetry rules [2].
© 2013 IEEE
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