Heterodyne detected Transient Grating UV/VIS-pump IR-probe measurements of energy transport through proteins

Halina Strzalka; Shabir Hassan; Paul M. Donaldson; Peter Hamm

doi:10.1364/ICUSD.2012.IM3D.7

Research in Optical Sciences
OSA Technical Digest (Optica Publishing Group, 2012),
paper IM3D.7
•https://doi.org/10.1364/ICUSD.2012.IM3D.7

Heterodyne detected Transient Grating UV/VIS-pump IR-probe measurements of energy transport through proteins

Halina Strzalka, Shabir Hassan, Paul M. Donaldson, and Peter Hamm

International Conference on Ultrafast Structural Dynamics 2012

Berlin Germany
19–21 March 2012
ISBN: 978-1-55752-939-8

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H. Strzalka, S. Hassan, P. M. Donaldson, and P. Hamm, "Heterodyne detected Transient Grating UV/VIS-pump IR-probe measurements of energy transport through proteins," in Research in Optical Sciences, OSA Technical Digest (Optica Publishing Group, 2012), paper IM3D.7.

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Abstract

The transient grating technique is applied to UV/VIS-pump IR-probe measurements to obtain a background free transient infrared signal. Two infrared probe beams, which are diffracted off a population grating in the sample, are heterodyne detected. The setup is passively phase stabilized. In comparison with conventional UV/VIS-pump IR-probe measurements the signal to noise is enhanced by a factor of 25. This enables us to follow IR transient absorption changes of single site labels in proteins in the sub-mM concentration range. As a first application we study the energy transport along proteins.

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