Practical aspects of super-resolution optical fluctuation image scanning microscopy (SOFISM)

Adrian Makowski; Gur Lubin; Ron Tenne; Aleksandra Sroda; Uri Rossman; Dan Oron; Radek Lapkiewicz

doi:10.1364/FIO.2020.FW7E.6

Frontiers in Optics / Laser Science
OSA Technical Digest (Optica Publishing Group, 2020),
paper FW7E.6
•https://doi.org/10.1364/FIO.2020.FW7E.6

Practical aspects of super-resolution optical fluctuation image scanning microscopy (SOFISM)

Adrian Makowski, Gur Lubin, Ron Tenne, Aleksandra Sroda, Uri Rossman, Dan Oron, and Radek Lapkiewicz

Frontiers in Optics 2020

Washington, DC United States
14–17 September 2020
ISBN: 978-1-943580-80-4

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Microscopic Imaging and Superesolution (FW7E)

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A. Makowski, G. Lubin, R. Tenne, A. Sroda, U. Rossman, D. Oron, and R. Lapkiewicz, "Practical aspects of super-resolution optical fluctuation image scanning microscopy (SOFISM)," in Frontiers in Optics / Laser Science, B. Lee, C. Mazzali, K. Corwin, and R. Jason Jones, eds., OSA Technical Digest (Optica Publishing Group, 2020), paper FW7E.6.

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Abstract

We reintroduce the concept of SOFISM and discuss its practical aspects. We demonstrate that supplying a confocal microscope with a SPAD array detector enables super-resolution imaging relying on fluorophore blinking within reasonable dwell times.

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