Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Mechanical properties of cellular nucleus characterized by Brillouin flow cytometry

Not Accessible

Your library or personal account may give you access

Abstract

Nucleus is the largest and usually stiffest organelle in a eukaryotic cell. Thus its mechanical properties is tightly related to various cell functions and activities. In this work, we developed a label-free flow cytometry platform to characterize the nuclear mechanics with the help of Brillouin microscopy, which is an all-optical technique so that can measure the stiffness of the nucleus in a non-contact, non-invasive and label-free manner. We demonstrate that Brillouin flow cytometry is able to detect physiologically-relevant changes in nuclear stiffness with enough sensitivity.

© 2017 Optical Society of America

PDF Article
More Like This
Brillouin Microscopy to Image Cell and Tissue Mechanical Properties

Giuliano Scarcelli
BoTu3A.1 Bio-Optics: Design and Application (BODA) 2017

High-Resolution Imaging of the Nuclear Envelope for Investigation of the Mechanical Influence of the Nucleus on Cellular Mechanics

Thorsten Kolb and Graeme Whyte
JT2A.33 Bio-Optics: Design and Application (BODA) 2013

Matched-Filter Compressive Imaging using a Deformable Mirror for Label-Free Flow Cytometry

William J Shain, Bennett B Goldberg, Thomas Bifano, and Jerome Mertz
ITu4E.1 Imaging Systems and Applications (IS) 2017

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved