Abstract

We have developed a Single Plane Illumination Microscope with ability to ablate single or groups of cells either through a photo-activated dye or by direct cell ablation. By integrating the ablation with heart synchronization we have ablated cells in a beating heart and observed the subsequent recovery within a zebrafish. Using a transgenic fish line containing the photo-activated “killer-red” compound we have used a Bessel beam, launched through the imaging arm of a SPIM system, to target killer-red expressing cells in the kidney. By integrating advanced optical techniques with transgenic methods the instrument developed has opened up a new route to study organ development, and repair.

© 2016 Optical Society of America