Abstract

We demonstrate a novel method of detection and differentiation of microobjects such as blood cells using amplitude and phase information from OCT signals applying dedicated microfluidic chip. The novel part of this method is that the optical identification is based on optical signal backscattered from a static scattering medium localized beneath the flowing/moving objects. In this study we use optical system combining phase-sensitive Fd-OCT with microfluidic device particularly designed for this study. In order to demonstrate the applicability of the method, we performed an experiment to differentiate between erythrocytes and leukocytes. Results of this differentiation are also presented.

© 2015 Optical Society of America