Photobleaching and correlation spectroscopy for in situ quantification of multi-labelled molecules on surfaces

Julius Sefkow-Werner; Julius Sefkow-Werner; Irene Wang; Catherine Picart; Elisa Migliorini; Antoine Delon

European Conferences on Biomedical Optics 2021 (ECBO)
OSA Technical Digest (Optica Publishing Group, 2021),
paper ES2A.4

Photobleaching and correlation spectroscopy for in situ quantification of multi-labelled molecules on surfaces

Julius Sefkow-Werner, Irene Wang, Catherine Picart, Elisa Migliorini, and Antoine Delon

European Conference on Biomedical Optics 2021

Munich Germany
20–24 June 2021
ISBN: 978-1-943580-95-8

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FLIM and Advanced Fluorescence Techniques (ES2A)

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J. Sefkow-Werner, I. Wang, C. Picart, E. Migliorini, and A. Delon, "Photobleaching and correlation spectroscopy for in situ quantification of multi-labelled molecules on surfaces," in European Conferences on Biomedical Optics 2021 (ECBO), OSA Technical Digest (Optica Publishing Group, 2021), paper ES2A.4.

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Abstract

We quantify multi-labelled streptavidin on a surface with un-known distribution of fluorophores in situ using correlation spectroscopy and photobleaching. This will allow the characterization of the functionalization of biomaterials for future regenerative medicine applications.

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