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Polarization-resolved SHG imaging of lamellar organization in keratoconic human corneas

Clothilde Raoux, Margaux Schmeltz, Marion Bied, Maged Alnawaiseh, Uwe Hansen, Gaël Latour, and Marie-Claire Schanne-Klein

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Abstract

We implement polarization-resolved second harmonic generation microscopy to characterize the orientation distribution of collagen lamellae in human cornea. We evidence a less ordered distribution in keratoconic corneas, in agreement with their deteriorated mechanical behavior.

© 2021 The Author(s)

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Structural imaging of keratoconic human corneas using polarization-resolved Second Harmonic Generation microscopy

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