Hydrogen peroxide level in tumor cells during cisplatin-induced apoptosis

Anastasiya S. Nerush; Kseniya M. Shсhukina; Irina V. Balalaeva; Anna G. Orlova

doi:10.1117/12.2527195

Medical Laser Applications and Laser-Tissue Interactions IX
SPIE Proceedings (Optica Publishing Group, 2019),
paper 11079_59
•https://doi.org/10.1117/12.2527195

Hydrogen peroxide level in tumor cells during cisplatin-induced apoptosis

Anastasiya S. Nerush, Kseniya M. Shсhukina, Irina V. Balalaeva, and Anna G. Orlova

European Conference on Biomedical Optics 2019

Munich Germany
23–25 June 2019
ISBN: 9781510628519

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Poster Session Tuesday - 11079 (PST11079)

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A. S. Nerush, K. M. Shсhukina, I. V. Balalaeva, and A. G. Orlova, "Hydrogen peroxide level in tumor cells during cisplatin-induced apoptosis," in Medical Laser Applications and Laser-Tissue Interactions IX, Vol. EB106 of SPIE Proceedings (Optica Publishing Group, 2019), paper 11079_59.

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Abstract

Using flow cytometry with a genetically encoded fluorescent sensor, vital dye and two types of apoptosis markers: PE Annexin V and TMRE, the participation of hydrogen peroxide (H₂O₂) in cisplatin-induced apoptosis was demonstrated. It was shown that cisplatin causes the increase of H₂O₂ level in early apoptotic cancer cells. Accumulation of H₂O₂ begins before the externalization of phosphatidylserine but after the loss of mitochondrial membrane potential. Scavenging of H₂O₂ prevents the cisplatin-induced apoptosis.

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