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Conferences > ECBO > 2019 > PSW11076 > 11076_72

Adaptive fluctuation imaging captures rapid subcellular dynamics

Ida S. Opstad, Florian Ströhl, Åsa B. Birgisdottir, Sebastián Maldonado, Trine Kalstad, Truls Myrmel, Krishna Agarwal, and Balpreet S. Ahluwalia

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Abstract

In this work we have explored the live-cell friendly nanoscopy method Multiple Signal Classification Algorithm (MUSICAL) for multi-colour imaging of various organelles and sub-cellular structures in the cardiomyoblast cell line H2c9. We have tested MUSICAL for fast (up to 230Hz), multi-colour time-lapse sequences of various sub-cellular structures (mitochondria, endoplasmic reticulum, microtubules, endosomes and nuclei) in living cells using low excitation-light dose. Challenges and opportunities with applying MUSICAL for studying rapid sub-cellular dynamics are discussed.

© 2019 SPIE/OSA

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