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Conferences > ECBO > 2019 > PSM11073 > 11073_67

Fluorescent imaging of polystyrene microspheres with cyanine dyes for immunofluorescence analysis

Pavel V. Shalaev, Ekaterina V. Bondina, Sergey A. Dolgushin, Victoria S. Semeykina, Natalya N. Sankova, and Ekaterina V. Parkhomchuk

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Abstract

Samples of liquid dispersions of 4 μm polystyrene microspheres with cyanine 5 and cyanine 5.5 dyes were synthesized and studied using developed experimental setup for fluorescence microscopy. It was shown the possibility of identification of microspheres with different dyes and different dye concentration in the image. The low coefficient of variation of fluorescence intensity in the image makes it possible to use a large number of types of spectrally encoded microspheres in the immunofluorescence analysis.

© 2019 SPIE/OSA

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