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X-ray irradiation effects on SH-SY5Y human neuroblastoma cells monitored by means of FTIR micro-spectroscopy

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Abstract

Fourier-Transform Infrared micro-spectroscopy (µFT-IR) was used for an in vitro investigation targeted to examine the effects on SH-SY5Y human neuroblastoma cells of X-rays irradiation. µFT-IR thanks to its ability in analyzing cells at a molecular level can be particularly useful in investigating the biochemical changes induced in protein, nucleic acid, lipid, and carbohydrate content of cells after irradiation by graded X-ray doses (0, 2, 4, 6, 8 and 10 Gy). Cell samples were fixed immediately (t0-cells) or 24h after (t24-cells) irradiation. A deconvolution procedure using Lorentzian curves was used for analyzing the collected spectra in order to highlight the changes due to the different treatment. Significant differences were evidenced in spectra from unexposed and exposed cells. Peaks related to important cell components (as Amide and DNA) show significant shifts depending on radiation dose and also the ratios between the intensity of selected bands are affected by the interaction with ionizing radiation. These changes are less evident in t24h-cells, thus suggesting the occurrence of repairing processes of the X-ray induced damage. The results of the present analysis can be helpful in order to develop innovative approaches to monitor radiation cancer radiotherapy outcome so as to reduce the overall radiation dose and minimize damage to the surrounding healthy cells, both aspects being of great importance in the field of radiation therapy.

© 2019 SPIE/OSA

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