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Rapid intraoperative margin assessment using multi-modal third-harmonic generation and three-photon fluorescence microscopy

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Abstract

Slide-free imaging of hematoxylin-eosin-stained whole mount tissues is demonstrated using multi-modal third harmonic generation and three photon fluorescence microscopy. As a result, the demonstrated microscopy shows promise for rapid margin assessment during surgery without compromising the contrast and features of conventional H&E histology.

© 2019 SPIE/OSA

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