Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Identification of azimuthal light scattering signatures to selectively track changes in subnuclear refractive index profile of epithelial cell models

Not Accessible

Your library or personal account may give you access

Abstract

We construct stochastically inhomogeneous epithelial cell models via simulation of Gaussian random fields; the extent and correlation length of subnuclear refractive index fluctuations are based on values quantified from high-resolution images of cervical tissue. We then employ the finite-difference time-domain method to simulate azimuth-resolved light scattering patterns of the constructed models. We process these two-dimensional patterns and calculate a series of Haralick features with the ultimate goal of identifying signatures that directly point to changes in subnuclear refractive index profile. Our results show that azimuthal contrast calculated over specific angular ranges is highly sensitive to the extent and correlation length of refractive index fluctuations. This metric is insensitive to changes in the overall size and mean refractive index of the constructed models, thereby allowing for selective tracking of changes in subnuclear refractive index variations.

© 2019 SPIE/OSA

PDF Article
More Like This
Influence of structural length-scale variations on azimuth-resolved light scattering patterns of inhomogeneous cell models

Dizem Arifler and Martial Guillaud
95370M European Conference on Biomedical Optics (ECBO) 2015

Assessing Epithelial Cell Nuclear Morphology with Azimuthal Light Scattering Spectroscopy

C. C. Yu, C. Lau, J. W. Tunnell, M. Hunter, M. Kalashnikov, C. Fang-Yen, S. F. Fulghum, K Badizadegan, R. R. Dasari, and M. S. Feld
TuD2 Biomedical Topical Meeting (BIOMED) 2006

Numerical analysis of dysplasia-associated changes in depth-dependent light scattering profile of cervical epithelium

Dizem Arifler, Calum MacAulay, Michele Follen, and Martial Guillaud
879809 European Conference on Biomedical Optics (ECBO) 2013

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved