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Conferences > ECBO > 2017 > PSIV > 104140N

Optimal Staining and Clearing Protocol for Whole Mouse Brain Vasculature Imaging with Light-sheet Microscopy

Antonino Paolo Di Giovanna, Alessandro Tibo, Ludovico Silvestri, Marie Caroline Müllenbroich, Irene Costantini, Leonardo Sacconi, Paolo Frasconi, and Francesco Saverio Pavone

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Abstract

Light-sheet microscopy enables whole mouse brain imaging in association with clearing methodologies. Here, we present a pipeline for optimal investigation of the vascular component, which offers improved image quality for morphological analysis.

© 2017 SPIE

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