Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Ultra-Sensitive Detection of Biomarker using Localized Surface Plasmon Resonance (LSPR) enhanced by ELISA

Not Accessible

Your library or personal account may give you access

Abstract

We demonstrate a highly sensitive detection of AFP (α-fetoprotein) protein (liver cancer marker) in human serum using the LSPR biosensor. Gold metal nanodot array (MNA) on a glass wafer were fabricated by UV nanoimprint lithography (NIL). After the NIL process using a film stamp and the removal of residual layer via oxygen plasma etching, metal films were deposited using an electron-beam evaporator, followed by the lift-off step. Consequently, the gold MNA was realized on 5-inch glass wafer and the pitch, diameter and height of MNA were 300nm, 150 nm and 20 nm, respectively. We employed observation of LSPR spectra via back-reflection, which provides a stable measurement of LSPR because a probe light does not pass a bio-sample. In addition, one channel among two flow channels was used a control channel, the MNA surface in which was modified with bovine serum albumin, not antibody. After antigen-antibody reaction, the enzyme/precipitation was employed on the MNA (Nano-ELISA). As a result, we could detect AFP in 50μL human serum with limit of detection (LOD) of 0.7 zeptomole (10−21 mole).

© 2015 SPIE

PDF Article
More Like This
Sensitive and Selective Detection of Prostate Specific Antigen beyond ELISA Using Photonic Crystal Nanolaser

Shoji Hachuda, Takumi Watanabe, Daichi Takahashi, and Toshihiko Baba
AM1J.3 CLEO: Applications and Technology (CLEO:A&T) 2015

Enhanced Sensitivity Using Liquid Crystals for Optical Fiber-Based Localized Surface Plasmon Resonance Sensor

Yongbin Lin, Yang Zou, Dan Ke, Jun Namkung, and Robert G. Lindquist
AWA4 Conference on Lasers and Electro-Optics: Applications (CLEO:A&T) 2010

On-chip Localized Surface Plasmon Resonance (LSPR) Sensing using Hybrid Plasmonic-photonic-fluidic Structures

Maysamreza Chamanzar, Zhixuan Xia, Ehsan Shah Hosseini, Siva Yegnanarayanan, and Ali Adibi
CTh4L.3 CLEO: Science and Innovations (CLEO:S&I) 2012

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved