Abstract

In this paper we present two-dimensional phantom measurements of fluorescence light distribution in the frequency domain and reconstruction of three-dimensional fluorophore distribution. An experimental set-up was built up with two dimensional laser scanning, intensity modulation with frequencies up to 1 GHz, and two-dimensional imaging of modulated fluorescence light. Stable phantoms were developed simulating mammary tissue to perform measurements in a backscattering geometry for a variety of cylindrical fluorescence sources with different diameters, fluorophore concentrations, and surface distances at different modulation frequencies. At first calculated fluorescence light distributions from Monte-Carlo simulations was compared to measured data. In a second step from tomographic data sets of calculated fluorescent light, three-dimensional tomographic reconstructions of fluorophore distribution were performed. Finally three-dimensional tomographic reconstructions of fluorophore distribution were performed from tomographic fluorescence measurements. We found good concurrence between measured and calculated fluorescence distribution. Synthetic and real tomographic reconstruction showed good localization but underestimated the depth of fluorophore distribution.

© 2011 OSA/SPIE